Field experiments were conducted during Kharif 2009 and 2010 at IARI farm, New Delhi to study the spectral reflectance characteristics of YMV susceptible (JS-335) and tolerant (Pusa-9814) varieties. In both the years, 90-100% leaves of the variety, JS-335 and 5-10% leaves of the variety, Pusa-9814 were infected with the disease in the field. In order to characterize spectral reflectance of healthy and YMV infected soybean crop, soybean leaves were collected from YMV-infected crop (JS-335) and healthy crop (Pusa-9814) and taken to laboratory for reflectance measurement under controlled condition. Leaf chlorophyll content was measured using DMSO method. Normalized Difference Vegetation Index (NDVI), Ratio Vegetation Index (RVI), Greeness Index (GI), Photochemical Reflectance Index (PRI) and Leaf Moisture Vegetation Index 1 (LMVI1) were computed and it was observed that NDVI was found to be useful in detecting yellow mosaic virus infected soybean.